VNIRTM 2X One-step RT-qPCR Probe Master Mix (no ROX) All Products Molecular Biology Products VNIR 118 Description VNIRTM 2X One-step RT-qPCR probe Master Mix (no ROX) is based on quantitative reverse transcription PCR (RT-qPCR) that uses RNA as the starting material. It offers a convenient master mix to convert RNA to DNA and quantify in a one-step reaction. The single-tube 2X formulation contains MMLV reverse transcriptase, dNTPs, Taq DNA polymerase, reaction buffer, MgCl2, KCl, and PCR enhancer/stabilizer facilitating easy reaction set up. The user needs only to add template, primer set, probe and water to the master mix to set up the RT-PCR reaction. This master mix is useful for efficient amplification of GC rich, low copy number genes from various template types and can be used up to 50 cycles. This master mix contains 2.5mM MgCl2 (1X final concentration). Cat. No.: VNIR118

VNIRTM 2X One-step RT-qPCR Probe Master Mix (ROX) All Products Molecular Biology Products VNIR 117 Description VNIRTM 2X One-step RT-qPCR probe Master Mix (ROX) is based on quantitative reverse transcription PCR (RT-qPCR) that uses RNA as the starting material. It offers a convenient master mix to convert RNA to DNA and quantify in a one-step reaction. The single-tube 2X formulation contains MMLV reverse transcriptase, dNTPs, ROX dye, Taq DNA polymerase, reaction buffer, MgCl2, KCl, and PCR enhancer/stabilizer facilitating easy reaction set up. The user needs only to add template, primer set, probe and water to the master mix to set up the RT-PCR reaction. This master mix is useful for efficient amplification of GC rich, low copy number genes from various template types and can be used up to 50 cycles. This master mix contains 2.5mM MgCl2 (1X final concentration). Cat. No.: VNIR117 Storage conditions -20°C (protect from light) References Please let us know if you publish your research using VNIRTM 2X One-step RT-qPCR probe Master Mix. We can cite your reference here. Note This formulation contains ROX as passive reference dye

VNIRTM Green 2X One-step RT-qPCR Master Mix (no ROX) All Products Molecular Biology Products VNIR 116 Description VNIRTM Green 2X One-step RT-qPCR Master Mix is based on quantitative reverse transcription PCR (RT-qPCR) that uses RNA as the starting material. It offers a convenient master mix to convert RNA to DNA and quantify in a one-step reaction. The single-tube 2X formulation contains MMLV reverse transcriptase, dNTPs, VNIR Green (equivalent to Sybr® Green), Taq DNA polymerase, reaction buffer, MgCl2, KCl, and PCR enhancer/stabilizer facilitating easy reaction set up. The user needs only to add the template, the primer set and water to the master mix to set up the RT-PCR reaction. This master mix is useful for efficient amplification of GC rich, low copy number genes from various template types and can be used up to 80 cycles. This master mix contains 2.5mM MgCl2 (1X final concentration). Cat. No.: VNIR116 Storage conditions -20°C (protect from light) Best before 24 months upon receiving References Please let us know if you publish your research using VNIRTM 2X One-step RT-qPCR Master Mix. We can cite your reference here. Note This formulation contain ROX as passive reference dye.

VNIRTM Green 2X One-step RT-qPCR Master Mix (ROX) All Products Molecular Biology Products VNIR 115 Description VNIRTM Green 2X One-step RT-qPCR Master Mix is based on quantitative reverse transcription PCR (RT-qPCR) that uses RNA as the starting material. It offers a convenient master mix to convert RNA to DNA and quantify in a one-step reaction. The single-tube 2X formulation contains MMLV reverse transcriptase, dNTPs, VNIR Green (equivalent to Sybr® Green), Taq DNA polymerase, reaction buffer, MgCl2, KCl, and PCR enhancer/stabilizer facilitating easy reaction set up. The user needs only to add the template, the primer set and water to the master mix to set up the RT-PCR reaction. This master mix is useful for efficient amplification of GC rich, low copy number genes from various template types and can be used up to 80 cycles. This master mix contains 2.5mM MgCl2 (1X final concentration). Cat. No.: VNIR115 Quality Control Assay Results Amplification efficiency >105 fold qPCR reproducibility Passed ds Endodeoxyribonuclease activity Not observed ss Exodeoxyribonuclease activity Not observed Self-priming activity Not observed Recommended reaction setup conditions Component Volume Final Concentration VNIR Green 2X One-step RT-qPCR Master Mix 10µl 1X Forward Primer – 10µM 0.5µl 500nM Reverse Primer – 10µM 0.5µl 500nM RNA Template Variable 50-400ng PCR grade water Variable – Total Volume per reaction 20 µl – *vortex briefly and spin down after addition of all components to ensure complete mixing Amplification Conditions Step Temperature Time Cycles RT 42°C 5-10 min 1 Enzyme Activation 95°C 3 min 35-45 Denaturation 95°C 30 sec Annealing, extension and read fluorescence Primers Tm 30 sec Storage conditions -20°C (protect from light) Best before 24 months upon receiving References Please let us know if you publish your research using VNIRTM Green 2X One-step RT-qPCR Master Mix. We can cite your reference here. Note This formulation doesn’t contain ROX.

VNIRTM Pro-Gel Stain-5000X All Products Molecular Biology Products VNIR 114 Description VNIRTM Pro-Gel Stain is a protein selective, orange emitting, ready-to-use fluorescent stain. The dye is tolerant to SDS and can be used in SDS-PAGE staining. The Stain is highly sensitive, detecting protein bands upto 2ng and exhibits low protein to protein variablity compared to other stains such as Coomassie ® brilliant blue (CBB) and silver staining techniques. The protein bands after staining can be visualized with a standard UV transilluminator or Blue light. Cat. No.: VNIR114 Concentration 5000X in DMSO. Appearance Reddish to Orange solution. Spectral Property Excitation: 300, 470nm Emission: 570nm Storage conditions -20°C (desiccate and protect from light) Stability 12 months upon receiving References Please let us know if you publish your research using VNIR TM Pro-Gel Stain. We can cite your reference here.