VNIRTM Protein Quantification Kit (fluorescence) All Products Assay Kits VNIR 307 Description VNIRTM Protein Quantification Kit (fluorescence) is designed for specific quantification of protein using a plate reader. VNIRTM Protein Quant. dye is highly specific to hydrophobic regions of the protein. The unique chemistry of the protein quantification reagent allows for the accurate detection of proteins in solution at concentrations between 50ng/mL and 10µg/mL. This assay kit shows less protein-to-protein variability than the Bradford assay. Cat. No.: VNIR307 Components & Storage Product Amount Concentration Storage VNIRTM Protein Quant. Dye – 50 assays 10µL 500X DMSO stock -20°C, in dark VNIRTM Protein Quant. Buffer 1mL 10X VNIRTM Protein Std. (BSA) 30µL 2mg/mL Protocol Prepare the 1X Protein Quant Buffer from 10X Protein Quant Buffer using distilled water. Prepare the 2X working solution by diluting the Protein Quant dye in Protein Quant Buffer, 1X. Prepare Protein standard in Protein Quant Buffer. Add 25µL of Protein Quant dye working solution and add 25µL protein standards or unknown samples. Allow plate to incubate at 90°C for 10 minutes in the dark. Add protein dye mixture to 96 well black plate along with blank (1X Protein Quant Buffer as blank). Read the plate at 474nm/580nm and analyse the data by linear regression fit. Spectral Property Excitation: 474nm; Emission: 580nm Storage conditions Store at -20°C (protect from light) References: Please let us know if you publish your research using VNIRTM Protein Quantification Kit. We can cite your reference here.

VNIRTM Protein Thermal Shift Assay Kit All Products Assay Kits VNIR 306 Description VNIRTM Thermal Shift Assay kit contains Thermal shift and buffer. The dye is a synthetic fluorescent probe with very high specificity to hydrophobic regions of protein. The probe formulation is developed to monitor the thermal profile of proteins and find the optimal parameters favouring the most stable conformations and also to study protein-ligand interactions by fluorescence intensity measurement using Real Time PCR systems in research and academic laboratories. VNIRTM Biotechnologies offers quick, easy, high throughput and cost efficient advantage to monitor the thermal profile of proteins in any finished product. Cat. No.: VNIR306 Components & Storage Product Amount Concentration Storage VNIR™ Thermal Shift Dye – 50 assays 10µL 200X DMSO stock -20℃, in dark VNIR™ Thermal Shift Buffer 1mL 10X 4-8℃ Protocol Thaw the buffer, Control protein and VNIR™ Thermal Shift Dye reagent on ice. Prepare about 1X Diluent Buffer by diluting in NFW. Prepare about 10X VNIR™ Thermal Shift Dye from 200X by diluting in 1X VNIR™ Thermal Shift buffer. Set up VNIRTM Thermal shift assay mix for 20µL per reaction: Component Volume per Reaction 10X VNIR™ Thermal Shift Dye 2 µL 1X VNIR™ Thermal Shift Buffer 5 µL Water + protein + buffer and/or buffer components 13 µL Total Up to 20 µL Pipette each reaction up and down 10 times to mix well. Seal the plate with transparent adhesive film, spin it at 4000rpm for 3-5 minutes, and then place it on ice. Set the qPCR reaction with below mentioned conditions in the real time PCR instrument. With inserting melt curve only, change the starting temperature to 20°C to 99°C in increments of 0.5°C for 5 seconds and select plate read. Select ROX channel as scan mode option, start the reaction. Load the plate, and then start the instrument run. Availability Cat.No.Pack size Quantity VNIR305.050 50 reactions VNIR305.100 100 reactions VNIR305.500 500 reactions References: Please let us know if you publish your research using VNIRTM Protein Thermal Shift Assay Kit. We can cite your reference here.

VNIRTM MTT Assay Kit (Cell Proliferation) All Products Assay Kits VNIR305 Description VNIRTM MTT assay kit is an non-radioactive, easy to use, high throughput assay for cell viability, proliferation, and cytotoxcity. The redox potential in active cells reduces the MTT to a insoluble purple formazan product, this can be solubilized and the color intensity can be read at 570nm using a microplate reader. There is no need to wash and harvest the cells before reading and can be performed in the same microplate. Cat. No.: VNIR305 Detection Method Colorimetric Absorbance max 570nm [end product] Sample type Adherent cells, suspension cells Components Number of reactions MTT Reagent Solubilization Solution 1 500 5 ml 20 ml 2 1000 10 ml 40 ml 3 2000 20 ml 80 ml Storage conditions -20°C (protect from light) Note -20°C (protect from light) References Please let us know if you publish your research using VNIRTM MTT assay kit. We can cite your article reference here.